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ldhcytotoxicity detection kit  (TaKaRa)


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    TaKaRa ldhcytotoxicity detection kit
    Ldhcytotoxicity Detection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1768 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytotoxicity+detection+kit/LDH+Cytotoxicity+Detection+Kit/10__1038_slash_s42003___026___10869___7-388-6-9
    Average 96 stars, based on 1768 article reviews
    ldhcytotoxicity detection kit - by Bioz Stars, 2026-10
    96/100 stars

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    Activity Assay:

    Article Title: Bryostatin enhances CD20 CAR-T therapy efficacy against B-cell lymphoma by overcoming trogocytosis-mediated antigen loss
    Article Snippet: .. The cytolytic activity of CAR-T cells was measured with the LDH-releasing assay using the Cytotoxicity Detection Kit (Takara-Bio). ..

    Concentration Assay:

    Article Title: Evaluation of respiratory toxicity and peritoneal mesothelioma induced by heat-treated and milled amosite following intratracheal and intraperitoneal injection in rats.
    Article Snippet: .. LDH concentration was measured using a Cytotoxicity Detection Kit (Takara Bio Inc., Tokyo, Japan) according to the manufacturer’s protocol. ..

    Cell Culture:

    Article Title: The probiotic yeast Saccharomyces boulardii CNCM I-745 prevents autophagy in macrophages and epithelial cells infected with Vibrio parahaemolyticus
    Article Snippet: .. LDH release into the cell culture supernatant was measured with Cytotoxicity Detection Kit (Takara Bio, Saint-Germain-en-Laye, France). ..



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    Image Search Results


    Baicalein preserves cell viability and morphology in lipopolysaccharide (LPS)-challenged H9c2 cardiomyoblasts. A, MTT cell viability assay. H9c2 cells were seeded at 1 × 10⁴ cells/well in 96-well plates and pretreated with baicalein (5 - 20 µM; 1 h), followed by LPS (1 µg/mL; 24 h). MTT reagent (5 mg/mL, Sigma M2128) was added for 4 h, formazan crystals were dissolved in DMSO, and absorbance was recorded at 570 nm (BioTek Synergy HTX). A calibration curve (1 – 8 × 10³ cells/well) was generated to validate linearity. Bars show mean ± SD. B, Inflammasome transcript kinetics (0 - 4 h). Total RNA was extracted using TRIzol™ (Invitrogen), converted to cDNA using the High-Capacity kit, and qPCR was performed with SYBR™ Green on a QuantStudio™ 5. Nlrp3, Il1b, and Il18 expression was evaluated from 0 - 4 h after LPS exposure to identify the mechanistic transcriptional peak. Relative abundance was calculated via 2⁻ΔΔCt using GAPDH as the reference. C, LDH release assay for membrane integrity. Supernatants were collected after 24 h of LPS stimulation, and LDH activity was quantified using the Takara MK401 kit. Absorbance at 490 nm was compared with the maximum lysis control to compute LDH release (%). D, Phase-contrast morphology. H9c2 cells were imaged on an Olympus CKX53 inverted microscope (20× objective) to assess rounding, shrinkage, and detachment following LPS exposure, and morphological preservation following baicalein pretreatment.

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Baicalein Protects H9c2 Cardiomyoblasts Against LPS-Induced Inflammatory Injury by Modulating the NF-κB/NLRP3 Inflammasome Pathway and Mitochondrial ROS

    doi: 10.5812/ijpr-169689

    Figure Lengend Snippet: Baicalein preserves cell viability and morphology in lipopolysaccharide (LPS)-challenged H9c2 cardiomyoblasts. A, MTT cell viability assay. H9c2 cells were seeded at 1 × 10⁴ cells/well in 96-well plates and pretreated with baicalein (5 - 20 µM; 1 h), followed by LPS (1 µg/mL; 24 h). MTT reagent (5 mg/mL, Sigma M2128) was added for 4 h, formazan crystals were dissolved in DMSO, and absorbance was recorded at 570 nm (BioTek Synergy HTX). A calibration curve (1 – 8 × 10³ cells/well) was generated to validate linearity. Bars show mean ± SD. B, Inflammasome transcript kinetics (0 - 4 h). Total RNA was extracted using TRIzol™ (Invitrogen), converted to cDNA using the High-Capacity kit, and qPCR was performed with SYBR™ Green on a QuantStudio™ 5. Nlrp3, Il1b, and Il18 expression was evaluated from 0 - 4 h after LPS exposure to identify the mechanistic transcriptional peak. Relative abundance was calculated via 2⁻ΔΔCt using GAPDH as the reference. C, LDH release assay for membrane integrity. Supernatants were collected after 24 h of LPS stimulation, and LDH activity was quantified using the Takara MK401 kit. Absorbance at 490 nm was compared with the maximum lysis control to compute LDH release (%). D, Phase-contrast morphology. H9c2 cells were imaged on an Olympus CKX53 inverted microscope (20× objective) to assess rounding, shrinkage, and detachment following LPS exposure, and morphological preservation following baicalein pretreatment.

    Article Snippet: - Lipopolysaccharide (LPS, E. coli O111:B4): Sigma-Aldrich, Cat. No. L2630 - Baicalein (≥ 98% purity): Sigma-Aldrich, Cat. No. 465119 - N-acetyl-L-cysteine (NAC): Sigma-Aldrich, Cat. No. A9165 - MTT reagent (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide): Sigma-Aldrich, Cat. No. M2128 - LDH Cytotoxicity Detection Kit: Takara Bio, Cat. No. MK401 - JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam, Cat. No. ab113850 - DCFH-DA ROS Detection Probe: Sigma-Aldrich, Cat. No. D6883 - TRIzolTM Reagent: Invitrogen, Cat. No. 15596026 - High-Capacity cDNA Reverse Transcription Kit: Applied Biosystems, Cat. No. 4368814 - SYBRTM Green PCR Master Mix: Applied Biosystems, Cat. No. 4367659 - Enzyme-linked immunosorbent assay (ELISA) Kits: Rat IL-1β ELISA: Abcam, Cat. No. ab255730

    Techniques: Viability Assay, Generated, SYBR Green Assay, Expressing, Lactate Dehydrogenase Assay, Membrane, Activity Assay, Lysis, Control, Inverted Microscopy, Preserving